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human peripheral blood lymphocyte separationmedium  (Beyotime)


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    Beyotime human peripheral blood lymphocyte separationmedium
    Human Peripheral Blood Lymphocyte Separationmedium, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 45 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+peripheral+blood+lymphocyte+separationmedium/Human+Peripheral+Blood+Lymphocyte+Separation+Medium/pm41084191-84-60-65
    Average 99 stars, based on 45 article reviews
    human peripheral blood lymphocyte separationmedium - by Bioz Stars, 2026-09
    99/100 stars

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    Isolation:

    Article Title: CD24 is a promising immunotherapeutic target for enhancing efficacy of third-generation EGFR-TKIs on EGFR-mutated lung cancer.
    Article Snippet: Phorbol 12- myristate 13-acetate (PMA) (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) was added to RPMI media to prime the THP-1 monocytes into macrophage-like cells (M0) for 48 h followed by washing the PMA off and a 24 h rest period in fresh media prior to exposure to polarizing cytokines. .. THP-1 cells were differentiated into M2a and M2c with exposure to 20 ng/mL interleukin (IL)-4 (Proteintech, Wuhan, Hubei, China) and IL-13 (Proteintech) or IL-10 (Proteintech) and transforming growth factor β1 (TGF-β1) (Proteintech) for another 48 h. To generate M2 macrophages from human peripheral blood mononuclear cells (PBMCs), we first isolated PBMCs from whole blood samples donated by healthy volunteers using Human Peripheral Blood Lymphocyte SeparationMedium (Beyotime).Next, we enrichCD14 positive monocytes from isolated PBMCs via Human CD14 MicroBeads UltraPure (Miltenyi Biotech, Cologne, Germany) according to the manufacturer’s instructions. .. Then the purified monocytes were cultured with 100 ng/mL recombinant human macrophage colony-stimulating factor (M-CSF) (Proteintech) for 7 days to differentiate them into M0 macrophages, and an additional 48 h with 20 ng/mL IL-4 (Proteintech) and IL-13 (Proteintech) for M2 macrophages.



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